|
|
| El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De. |
| Abstract Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia. Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301 |
| |
